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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
02/01/2017 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
CASTILLO, A.; GAIERO, P.; LÓPEZ CARRO, B.; VILARO, F. |
Afiliación : |
ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; P. GAIERO, Universidad de la República (UdelaR)/ Facultad de Agronomía; B. LÓPEZ CARRO, SECIF-IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Gametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
Plant Tissue Culture and Biotechnology, 2016, v. 26 (2): 159-173. |
DOI : |
10.3329/ptcb.v26i2.30566 |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding. |
Palabras claves : |
ANTHER CULTURE; DOUBLE HAPLOID; FLOW CYTOMETRY; HAPLOID. |
Thesagro : |
GERMOPLASMA; PAPA; SOLANUM COMMERSONII. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13501/1/30566-Article-Text-109802-1-10-20161210.pdf
https://www.banglajol.info/index.php/PTCB/article/view/30566
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Marc : |
LEADER 01724naa a2200253 a 4500 001 1056407 005 2019-10-15 008 2016 bl uuuu u00u1 u #d 024 7 $a10.3329/ptcb.v26i2.30566$2DOI 100 1 $aCASTILLO, A. 245 $aGametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding.$h[electronic resource] 260 $c2016 520 $aABSTRACT. The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding. 650 $aGERMOPLASMA 650 $aPAPA 650 $aSOLANUM COMMERSONII 653 $aANTHER CULTURE 653 $aDOUBLE HAPLOID 653 $aFLOW CYTOMETRY 653 $aHAPLOID 700 1 $aGAIERO, P. 700 1 $aLÓPEZ CARRO, B. 700 1 $aVILARO, F. 773 $tPlant Tissue Culture and Biotechnology, 2016$gv. 26 (2): 159-173.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha actual : |
21/02/2014 |
Actualizado : |
14/06/2018 |
Tipo de producción científica : |
Documentos |
Autor : |
MENDEZ, R.; ROEL, A.; FUREST, J. |
Afiliación : |
RAMON FELIPE MENDEZ LARROSA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALVARO ROEL DELLAZOPPA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSE MARIA FUREST CROCCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Comportamiento de las principales variables climáticas en la zafra 2001/02, zona Este. |
Complemento del título : |
Ecofisiología del cultivo. |
Fecha de publicación : |
2002 |
Fuente / Imprenta : |
ln: INIA TREINTA Y TRES. Arroz: resultados experimentales 2001-2002. Treinta y Tres (Uruguay): INIA, 2002. |
Páginas : |
cap. 2, p. 1-8. |
Serie : |
(INIA Serie Actividades de Difusión; 292) |
Idioma : |
Español |
Thesagro : |
ARROZ; CULTIVO; FACTORES CLIMATICOS; URUGUAY. |
Asunto categoría : |
P40 Meteorología y climatología |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/10268/1/Ad-292-cap-2-p.1-8.pdf
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Marc : |
LEADER 00646naa a2200205 a 4500 001 1031404 005 2018-06-14 008 2002 bl uuuu u00u1 u #d 100 1 $aMENDEZ, R. 245 $aComportamiento de las principales variables climáticas en la zafra 2001/02, zona Este. 260 $c2002 300 $acap. 2, p. 1-8. 490 $a(INIA Serie Actividades de Difusión; 292) 650 $aARROZ 650 $aCULTIVO 650 $aFACTORES CLIMATICOS 650 $aURUGUAY 700 1 $aROEL, A. 700 1 $aFUREST, J. 773 $tln: INIA TREINTA Y TRES. Arroz: resultados experimentales 2001-2002. Treinta y Tres (Uruguay): INIA, 2002.
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